https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 Gauging the skin resident Leishmania parasites through a loop mediated isothermal amplification (LAMP) assay in post-kala-azar dermal leishmaniasis https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:52460 Wed 11 Oct 2023 15:02:47 AEDT ]]> Evaluation of real-time PCR for diagnosis of Post-kala-azar dermal leishmaniasis in endemic foci of Bangladesh https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:37513 Leishmania donovan body (LDB) and LD DNA, respectively. Results: Real-time PCR detected 83 cases among all suspected PKDL patients, with an encouraging sensitivity of 91.2% (83.4%-96.1%), whereas microscopy showed 50.6% (39.9%-61.2%) sensitivity. Among all suspected PKDL cases, 42 cases were positive in both microscopy and qPCR, whereas 41 cases were detected as positive through qPCR only. Conclusions: This study provides evidence that real-time PCR is a promising tool for diagnosis of PKDL in endemic regions. In addition to diagnosis, the quantitative ability of this method could be further exploited for after-treatment prognosis and cure assessment of PKDL cases.]]> Mon 05 Dec 2022 13:50:01 AEDT ]]>